In vivo biocompatibility and degradability of a novel injectable-chitosan-based implant

被引:459
作者
Mi, FL
Tan, YC
Liang, HF
Sung, HW [1 ]
机构
[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 30013, Taiwan
[2] Chinese Naval Acad, Div Chem, Dept Math Phys & Chem, Kaohsiung 813, Taiwan
关键词
chitosan; microspheres; genipin; glutaraldehyde; degradation; biocompatibility;
D O I
10.1016/S0142-9612(01)00094-1
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A novel injectable-chitosan-based delivery system with low cytotoxicity was fabricated in the study. The chitosan microspheres with small particle size, low crystallinity and good sphericity were prepared by a spray-drying method followed by treating with a crosslinker. In the study, a naturally occurring crosslinking reagent (genipin), which has been used in herbal medicine and in the production of food dyes, was used to crosslink the chitosan microspheres. The glutaraldehyde-crosslinked counterparts were used as a control. Histological study of the genipin-crosslinked chitosan microspheres injected intramuscularly into the skeletal muscle of a rat model showed a less inflammatory reaction than its glutaraldehyde-crosslinked counterparts. The results of the scanning electron microscopic examination indicated that the glutaraldehyde-crosslinked chitosan microspheres retrieved at 12-week postoperatively were already degraded into a loose and porous structure. However, the degradation of the genipin-crosslinked chitosan microspheres was not significant after 20 weeks of implantation. The results of the study demonstrated that the genipin-crosslinked chitosan microspheres have a superior biocompatibility and a slower degradation rate than the glutaraldehyde-crosslinked chitosan microspheres. Accordingly, the genipin-crosslinked chitosan microspheres may be a suitable polymeric carrier for long-acting injectable drug delivery. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:181 / 191
页数:11
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