Glucose-sensitive neurons in the rat arcuate nucleus contain neuropeptide Y

被引：165

作者：

Muroya, S

Yada, T ^{[1
]}

Shioda, S

Takigawa, M

机构：

[1] Kagoshima Univ, Sch Med, Dept Physiol, Kagoshima 8908520, Japan

[2] Kagoshima Univ, Sch Med, Dept Psychiat, Kagoshima 8908520, Japan

[3] Showa Univ, Sch Med, Dept Anat, Tokyo 1428555, Japan

[4] Natl Inst Physiol Sci, Lab Intracellular Metab, Okazaki, Aichi 4448585, Japan

来源：

NEUROSCIENCE LETTERS | 1999年 / 264卷 / 1-3期

关键词：

arcuate nucleus; glucose-sensitive neuron; hypothalamus; neuropeptide Y; cytosolic Ca2+; immunocytochemistry; feeding centers;

D O I：

10.1016/S0304-3940(99)00185-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Glucose is known to regulate the activity of the hypothalamic feeding centers. Neuropeptide Y (NPY)-containing neurons in the hypothalamic arcuate nucleus (ARC) have been implicated in the stimulation of feeding. We examined the presence of glucose-sensitive neurons in the ARC and their coincidence with NPY-containing neurons. Cytosolic Ca2+ concentration ([C-a2+](i)) in single ARC neurons isolated from rat hypothalamus was measured with fura-e fluorescence imaging; the cells were then stained immunocytochemically with an anti-NPY antiserum. Lowering the glucose concentration from 10 to 1 mM increased [Ca2+](i) in 36 out of 180 neurons (20%), the majority of which (34 neurons, 94%) were immunoreactive for NPY. In conclusion, the ARC contains glucose-sensitive NPY-containing neurons. The suggested role of these neurons is to transduce a reduction in the glucose concentration in the brain to the release of NPY and, subsequently, stimulation of feeding. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：113 / 116

页数：4

共 18 条

[1] SPECIFIC-INHIBITION OF ENDOGENOUS NEUROPEPTIDE-Y SYNTHESIS IN ARCUATE NUCLEUS BY ANTISENSE OLIGONUCLEOTIDES SUPPRESSES FEEDING-BEHAVIOR AND INSULIN-SECRETION [J].