A novel quantum dot-based fluoroimmunoassay method for detection of Enrofloxacin residue in chicken muscle tissue

被引：88

作者：

Chen, Junxia ^{[1
]}

Xu, Fei ^{[1
]}

Jiang, Haiyang ^{[1
]}

Hou, Yali ^{[2
]}

Rao, Qinxiong ^{[3
]}

Guo, Pengju ^{[4
]}

Ding, Shuangyang ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residue, Beijing 100094, Peoples R China

[2] Chongqing Inst Vet Drug & Feed Inspect, Chongqing 401147, Peoples R China

[3] Shanghai Acad Agr Sci, Inst Agr Food Standards & Testing, Shanghai 201100, Peoples R China

[4] Australian Anim Hlth Lab, CSIRO, Livestock Ind, Geelong, Vic, Australia

来源：

FOOD CHEMISTRY | 2009年 / 113卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Enrofloxacin; Quantum dots; Chicken muscle; Fluoro mmunoassay; LINKED-IMMUNOSORBENT-ASSAY; LIQUID-CHROMATOGRAPHY; ANTIBODY; ELISA; ANTIBIOTICS; EGGS;

D O I：

10.1016/j.foodchem.2008.08.006

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A rapid indirect competitive fluorescence-linked immunosorbent assay (cFLISA) based on quantum Clots (QDs) as the fluorescent marker has been developed for the detection of Enrofloxacin (ENR) in chicken Muscle tissue. The end-point fluorescent detection system was carried out using QDs conjugated with goat anti-mouse secondary antibody. The cFLISA method allowed for ENR determination in a liner working range of 1-100 ng mL(-1) with the 50% inhibition Value (IC50) of 8.3 ng mL(-1) and the limit of detection (LOD) of 2.5 ng mL(-1). The recoveries for chicken muscle samples spiked with ENR at levels of 50-200 mu g kg(-1) ranged from 81% to 94% with coefficients of variation (CV) of 10-13%. In real chicken tissue sample analysis, the results of cFLISA were similar to those obtained from an indirect competitive enzyme-linked immunosorbent assay (cELISA) to a high performance liquid chromatography method (HPLC), which indicated that cFLISA is Suitable as screening method for the monitoring of veterinary drug residues. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1197 / 1201

页数：5

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