Preparative isolation of the eutomer of cyclothiazide by high-performance liquid chromatography on a cellulose-derived chiral stationary phase with toluene-acetone as the mobile phase

被引：14

作者：

Oliveros, L

Minguillon, C

Serkiz, B

Meunier, F

Volland, JP

Cordi, AA

机构：

[1] UNIV BARCELONA,FAC FARM,QUIM FARMACEUT LAB,E-08028 BARCELONA,SPAIN

[2] INST RECH SERVIER,F-92150 SURESNES,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 729卷 / 1-2期

关键词：

chiral stationary phases; LC; enantiomer separation; preparative chromatography; diastereomer separation; cyclothiazide;

D O I：

10.1016/0021-9673(95)01036-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enantiomeric pair containing the eutomer of cyclothiazide can be resolved by HPLC on commercially available cellulose-derived chiral stationary phases (CSPs). However, the poor solubility of cyclothiazide in solvents compatible with this kind of CSP makes this separation difficult on a preparative scale. This operation was achieved with a CSP constituted by a 3,5-dimethylphenylcarbamate/10-undecenoate derivative of cellulose fixed on allylsilica gel. This CSP is resistant to all the polar solvents usually applied in HPLC.

引用

页码：29 / 32

页数：4

共 6 条

[1] IDENTIFICATION AND CHARACTERIZATION OF THE ISOMERS OF CYCLOTHIAZIDE RESPONSIBLE FOR POTENTIATING AMPA CURRENT [J].