A role for a rat homolog of staufen in the transport of RNA to neuronal dendrites

被引：185

作者：

Tang, SJ ^{[1
]}

Meulemans, D ^{[1
]}

Vazquez, L ^{[1
]}

Colaco, N ^{[1
]}

Schuman, E ^{[1
]}

机构：

[1] CALTECH, Howard Hughes Med Inst, Div Biol, Pasadena, CA 91125 USA

来源：

NEURON | 2001年 / 32卷 / 03期

关键词：

D O I：

10.1016/S0896-6273(01)00493-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

RNAs are present in dendrites and may be used for local protein synthesis in response to synaptic activity. To begin to understand dendritic RNA targeting, we cloned a rat homolog of staufen, a Drosophila gene that participates in mRNA targeting during development. In hippocampal neurons, rat staufen protein displays a microtubule-dependent somatodendritic distribution pattern that overlaps with dendritic RNAs. To determine whether r-staufen is required for dendritic RNA targeting, we constructed a mutant version containing the RNA binding domains (stau-RBD) but lacking the C-terminal portion potentially involved in dendritic targeting. Stau-RBD expression was restricted to the cell bodies and proximal dendrites. Expression of stau-RBD significantly decreased, while overexpression of wild-type r-staufen increased, the amount of dendritic mRNA. Taken together, these results suggest that the rat staufen protein plays an important role in the delivery of RNA to dendrites.

引用

页码：463 / 475

页数：13