IL-10-secreting T cells from HIV-infected pregnant women downregulate HIV-1 replication: effect enhanced by antiretroviral treatment

被引：25

作者：

Bento, Cleonice A. M. ^{[1
]}

Hygino, Joana ^{[1
]}

Andrade, Regis M. ^{[1
]}

Saramago, Carmen S. M. ^{[1
]}

Silva, Renato G. ^{[1
]}

Silva, Agostinho A. L. ^{[1
]}

Linhares, Ulisses C. ^{[2
]}

Brindeiro, Rodrigo ^{[3
]}

Tanuri, Amilcar ^{[3
]}

Rosenzwajg, Michelle ^{[4
]}

Klatzmann, David ^{[4
]}

Andrade, Arnaldo F. B. ^{[5
]}

机构：

[1] Univ Fed Rio de Janeiro, Dept Microbiol & Parasitol, BR-20261040 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Dept Anat, BR-20261040 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Dept Genet, BR-20261040 Rio De Janeiro, Brazil

[4] Univ Paris 06, CNRS, UPCM UMR 7087, Lab Biol & Therapeut Pathol Immunitaires, Paris, France

[5] Univ Estado Rio De Janeiro, Dept Microbiol, Rio De Janeiro, Brazil

来源：

AIDS | 2009年 / 23卷 / 01期

关键词：

antiretroviral therapy; cytokines; HIV; interleukin-10; pregnancy; tumor necrosis factor-alpha; HUMAN-IMMUNODEFICIENCY-VIRUS; NECROSIS-FACTOR-ALPHA; IMMUNE ACTIVATION; CHRONIC HEPATITIS; HUMAN MACROPHAGES; VIRAL-LOAD; IN-VITRO; INTERLEUKIN-10; CD4(+); TYPE-1;

D O I：

10.1097/QAD.0b013e328317461e

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Objective: This study aimed to evaluate the impact of pregnancy-related immune events on the HIV-1 replication and to analyze their relationship with the risk of vertical transmission. Methods: The peripheral blood from HIV-1-infected pregnant women who controlled (G1) or not controlled (G2) their plasma viral load was drawn, and the plasma and the T cells were obtained. The T-cell cultures were activated in vitro with anti-CD3 and anti-CD28, and the proliferation and cytokine production profile were evaluated after 3 days of incubation. The in-vitro HIV-1 replication was measured in culture supernatants in the seventh day following stimulation. The cytokines were also analyzed in the plasma. Results: Our results demonstrated a lower T-cell proliferation and a lower interleukin-1 beta, tumor necrosis factor-alpha and interferon-gamma production in polyclonally activated T-cell cultures from G1 patients, when compared with G2. Furthermore, high levels of interleukin-10 were produced both systemically and by activated T-cell cultures from G1 patients. Interestingly, the neutralization of endogenous interleukin-10 bby anti-interleukin-10 monoclonal antibody elevated both the inflammatory cytokines' release and the HIV-1 replication in the polyclonally activated T-cell cultures from G1 patients. Additionally, the maternal antiretroviral treatment significantly enhanced the systemic interleukin-10 production. Finally, the higher systemic interleukin-10 levels were inversely correlated with vertical virus transmission risk. Conclusion: These results indicate that a high tendency of pregnant women to produce interleukin-10 can help them control the HIV-1 replication, and this can reduce the risk of vertical transmission. Furthermore, our data suggest a role for maternal antiretroviral treatment in enhancing this phenomenon. (C) 2009 Wolters Kluwer Health vertical bar Lippincolt Williams & Wilkins

引用

页码：9 / 18

页数：10

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