An approach to rapid protein crystallization using nanodroplets

被引:211
作者
Santarsiero, BD
Yegian, DT
Lee, CC
Spraggon, G
Gu, J
Scheibe, D
Uber, DC
Cornell, EW
Nordmeyer, RA
Kolbe, WF
Jin, J
Jones, AL
Jaklevic, JM
Schultz, PG
Stevens, RC
机构
[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[2] Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USA
[3] EO Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA
[4] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[5] Joint Ctr Struct Genom, La Jolla, CA 92037 USA
关键词
D O I
10.1107/S0021889802001474
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An approach that enables up to a two order of magnitude reduction in the amount of protein required and a tenfold reduction in the amount of time required for vapor-diffusion protein crystallization is reported. A prototype high-throughput automated system was used for the production of diffraction-quality crystals for a variety of proteins from a screen of 480 conditions using drop volumes as small as 20 nL. This approach results in a significant reduction in the time and cost of protein structure determination, and allows for larger and more efficient screens of crystallization parameter space. The ability to produce diffraction-quality crystals rapidly with minimal quantities of protein enables high-throughput efforts in structural genomics and structure-based drug discovery.
引用
收藏
页码:278 / 281
页数:4
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