Conformational preferences of a chimeric peptide HIV-1 immunogen from the C4-V3 domains of gp120 envelope protein of HIV-1 CANOA based on solution NMR: Comparison to a related immunogenic peptide from HIV-1 RF

被引:29
作者
Vu, HM
deLorimier, R
Moody, MA
Haynes, BF
Spicer, LD
机构
[1] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710
[2] DUKE UNIV,MED CTR,DEPT MED,DURHAM,NC 27710
[3] DUKE UNIV,MED CTR,DEPT IMMUNOL,DURHAM,NC 27710
[4] DUKE UNIV,MED CTR,DEPT RADIOL,DURHAM,NC 27710
关键词
D O I
10.1021/bi952665x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A critical problem to overcome in HIV vaccine design is the variability among HIV strains, One strategy to solve this problem is the construction of multicomponent immunogens reflective of common HIV motifs, Currently, it is not known if these motifs should be based primarily on amino acid sequence or higher-order structure of the viral proteins or a combination of the two. In this paper, we report NMR-derived solution conformations for a synthetic peptide taken from the C4 and V3 domains of HIV-1 CAN0A gp120 envelope protein. This peptide, designated T1-SP10CAN0(A), is compared to a recently reported C4-V3 peptide, T1-SP10RF(A) from the HIV-1 RF strain [de Lorimier et al. (1994) Biochemistry 33, 2055-2062], in terms of conformational features and immune responses in mice [Haynes er al. (1995) AIDS Res. Hum. Retroviruses 11, 211-221]. The T1 segment of 16 amino acids from the gp120 C4 domain is identical in both peptides and exhibits nascent helical character. The SP10 region, taken from the gp120 V3 loop, differs from that of T1-SP10RF(A) in both sequence and conformations, A reverse turn is observed at the conserved GPGX sequence. The rest of the SP10 domain is extended with the exception of the last three residues which show evidence for a helical arrangement. Modeling of the turn region of the T1-SP10CAN0(A) peptide shows exposure of a continuous apolar stretch of side chains similar to that reported in the crystal structure of a V3 peptide from HIV-1 MN complexed with a monoclonal antibody [Rini er al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 6325-6329]. This hydrophobic patch is interrupted by a charged Lys residue in the T1-SP10RF(A) peptide. This observation suggests that the HIV-1 CAN0A and HIV-1 RF C4-V3 peptides can induce widely different anti-HIV antibodies, consistent with immunogenic results.
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页码:5158 / 5165
页数:8
相关论文
共 64 条
[61]  
Wuthrich K., 1986, EUROPHYS NEWS, V17, P11
[62]   SOLUTION CONFORMATION OF A PEPTIDE CORRESPONDING TO THE PRINCIPAL NEUTRALIZING DETERMINANT OF HIV-1(IIIB) - A 2-DIMENSIONAL NMR-STUDY [J].
ZVI, A ;
HILLER, R ;
ANGLISTER, J .
BIOCHEMISTRY, 1992, 31 (30) :6972-6979
[63]  
ZVI A, 1995, EUR J BIOCHEM, V229, P178, DOI 10.1111/j.1432-1033.1995.0178l.x
[64]   IMMUNODOMINANCE AND ANTIGENIC VARIATION OF THE PRINCIPAL NEUTRALIZATION DOMAIN OF HIV-1 [J].
ZWART, G ;
LANGEDIJK, H ;
VANDERHOEK, L ;
DEJONG, JJ ;
WOLFS, TFW ;
RAMAUTARSING, C ;
BAKKER, M ;
DERONDE, A ;
GOUDSMIT, J .
VIROLOGY, 1991, 181 (02) :481-489