Fast-Scan Controlled-Adsorption Voltammetry for the Quantification of Absolute Concentrations and Adsorption Dynamics

被引：81

作者：

Atcherley, Christopher W. ^{[1
]}

Laude, Nicholas D. ^{[1
]}

Parent, Kate L. ^{[1
]}

Heien, Michael L. ^{[1
]}

机构：

[1] Univ Arizona, Dept Chem & Biochem, Tucson, AZ 85721 USA

来源：

LANGMUIR | 2013年 / 29卷 / 48期

关键词：

CARBON-FIBER MICROELECTRODES; CYCLIC VOLTAMMETRY; DOPAMINE; BRAIN; SENSITIVITY; CONVOLUTION; ELECTRODES; SEROTONIN; KINETICS; RELEASE;

D O I：

10.1021/la402686s

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Fast-scan cyclic voltammetry has depended on background subtraction to quantify small changes in neurotransmitter concentration. Because of this requirement, measurements of absolute concentrations using fast-scan cyclic voltammetry have been limited. Here we develop and characterize fast-scan controlled-adsorption voltammetry (FSCAV), which enables direct measurements of absolute concentrations in vitro without the use of flow injection to change the concentration. This enables probing the diffusion-controlled adsorption dynamics of biogenic amines and other adsorbing species. An implicit finite-difference model of mass-transport-limited adsorption was developed and is in agreement with experimental results. Optimization of FSCAV yielded a sensitivity of 81 +/- 11 nA/mu M for dopamine, corresponding to a limit of detection of 3.7 +/- 0.5 nM. Through the combination of novel instrumentation and validated computer simulations, we show that FSCAV is an important measurement tool that can be used to determine absolute concentrations and study mass-transport-limited adsorption.

引用

页码：14885 / 14892

页数：8

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