High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

被引：657

作者：

Boutet, Sebastien ^{[1
]}

Lomb, Lukas ^{[2
,3
]}

Williams, Garth J.

Barends, Thomas R. M. ^{[2
,3
]}

Aquila, Andrew ^{[4
]}

Doak, R. Bruce ^{[5
]}

Weierstall, Uwe ^{[5
]}

DePonte, Daniel P. ^{[4
]}

Steinbrener, Jan ^{[2
,3
]}

Shoeman, Robert L. ^{[2
,3
]}

Messerschmidt, Marc

Barty, Anton ^{[4
]}

White, Thomas A. ^{[4
]}

Kassemeyer, Stephan ^{[2
,3
]}

Kirian, Richard A. ^{[5
]}

Seibert, M. Marvin

Montanez, Paul A.

Kenney, Chris

Herbst, Ryan

Hart, Philip

Pines, Jack

Haller, Gunther

Gruner, Sol M. ^{[6
,7
]}

Philipp, Hugh T. ^{[6
]}

Tate, Mark W. ^{[6
]}

Hromalik, Marianne ^{[8
]}

Koerner, Lucas J. ^{[9
]}

van Bakel, Niels ^{[10
]}

Morse, John ^{[11
]}

Ghonsalves, Wilfred

Arnlund, David ^{[12
]}

Bogan, Michael J. ^{[1
]}

Caleman, Carl ^{[4
]}

Fromme, Raimund ^{[13
]}

Hampton, Christina Y. ^{[1
]}

Hunter, Mark S. ^{[13
]}

Johansson, Linda C. ^{[12
]}

Katona, Gergely ^{[12
]}

Kupitz, Christopher ^{[13
]}

Liang, Mengning ^{[4
]}

Martin, Andrew V. ^{[4
]}

Nass, Karol

Redecke, Lars ^{[14
,15
,16
]}

Stellato, Francesco ^{[4
]}

Timneanu, Nicusor ^{[17
]}

Wang, Dingjie ^{[5
]}

Zatsepin, Nadia A. ^{[5
]}

Schafer, Donald

Defever, James

Neutze, Richard ^{[12
]}

机构：

[1] SLAC Natl Accelerator Lab, PULSE Inst, Menlo Pk, CA 94025 USA

[2] Max Planck Inst Med Res, D-69120 Heidelberg, Germany

[3] Ctr Free Electron Laser Sci, Max Planck Adv Study Grp, D-22607 Hamburg, Germany

[4] Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany

[5] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[6] Cornell Univ, Dept Phys, Atom & Solid State Phys Lab, Ithaca, NY 14853 USA

[7] Cornell Univ, Cornell High Energy Synchrotron Source, Wilson Lab, Ithaca, NY 14853 USA

[8] SUNY Coll Oswego, Oswego, NY 13126 USA

[9] Johns Hopkins Univ, Appl Phys Lab, Laurel, MD 20723 USA

[10] Natl Inst Subatom Phys, Nikhef, NL-1098 XG Amsterdam, Netherlands

[11] European Synchrotron Radiat Facil, F-38043 Grenoble, France

[12] Univ Gothenburg, Dept Chem & Mol Biol, SE-40530 Gothenburg, Sweden

[13] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

[14] Univ Hamburg, Inst Biochem & Mol Biol, Joint Lab Struct Biol Infect & Inflammat, D-22761 Hamburg, Germany

[15] Univ Lubeck, Inst Biochem, DESY, Hamburg, Germany

[16] Univ Lubeck, German Ctr Infect Res, D-23538 Lubeck, Germany

[17] Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, SE-75124 Uppsala, Sweden

来源：

SCIENCE | 2012年 / 337卷 / 6092期

基金：

瑞典研究理事会; 美国国家科学基金会;

关键词：

DIFFRACTION DATA; LYSOZYME;

D O I：

10.1126/science.1217737

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.

引用

页码：362 / 364

页数：3

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