Determination of nefazodone and its pharmacologically active metabolites in human blood plasma and breast milk by high-performance liquid chromatography

被引：13

作者：

Dodd, S ^{[1
]}

Buist, A ^{[1
]}

Burrows, GD ^{[1
]}

Maguire, KP ^{[1
]}

Norman, TR ^{[1
]}

机构：

[1] Univ Melbourne, Austin & Repatriat Med Ctr, Dept Psychiat, Melbourne, Vic 3084, Australia

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 730卷 / 02期

关键词：

nefazodone;

D O I：

10.1016/S0378-4347(99)00225-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the determination of nefazodone and its active metabolites hydroxynefazodone, the dione BMS-180492 and m-chlorophenylpiperazine in blood plasma and expressed human milk based on reversed-phase highperformance liquid chromatography. Measurements were performed on drug-free plasma and expressed human milk spiked with nefazodone and metabolites to prepare and validate standard curves and specimens collected from nursing mothers. Parent drug and metabolites were separated from the biological matrices by solid-phase extraction using CERTIFY columns. Chromatographic separation was achieved with a C-18 column and compounds were detected by their absorbance at 205 nm. Trazodone was used as an internal standard. The assay was validated for each analyte in the concentration range 200 to 1200 ng/ml. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：249 / 255

页数：7

共 7 条

[1] A study of the effect of age and gender on the pharmacokinetics of nefazodone after single and multiple doses [J].

Barbhaiya, R ;

Buch, AB ;

Greene, DS .

JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY, 1996, 16 (01) :19-25

[2] Nefazodone pharmacokinetics: Assessment of nonlinearity, intra-subject variability and time to attain steady-state plasma concentrations after dose escalation and de-escalation [J].

Barbhaiya, RH ;

Shukla, UA ;

Chaikin, P ;

Greene, DS ;

Marathe, PH .

EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 1996, 50 (1-2) :101-107

[3] Behavioral, physiological and neuroendocrine responses in healthy volunteers to m-chlorophenylpiperazine (m-CPP) with and without ondansetron pretreatment [J].

Broocks, A ;

Briggs, NC ;

Pigott, TA ;

Hill, JL ;

Canter, SK ;

Tolliver, TJ ;

Baldemore, D ;

Murphy, DL .

PSYCHOPHARMACOLOGY, 1997, 130 (02) :91-103

[4]

FEIGHNER JP, 1989, PSYCHOPHARMACOL BULL, V25, P219

[5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF NEFAZODONE AND ITS METABOLITES IN HUMAN PLASMA USING LABORATORY ROBOTICS [J].

FRANC, JE ;

DUNCAN, GF ;

FARMEN, RH ;

PITTMAN, KA .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 570 (01) :129-138

[6] DETERMINATION OF NEFAZODONE AND ITS METABOLITES IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION [J].

FRANKLIN, M .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (11-12) :1109-1113

[7] NONLINEAR PHARMACOKINETICS OF NEFAZODONE AFTER ESCALATING SINGLE AND MULTIPLE ORAL DOSES [J].

KAUL, S ;

SHUKLA, UA ;

BARBHAIYA, RH .

JOURNAL OF CLINICAL PHARMACOLOGY, 1995, 35 (08) :830-839

← 1 →