Cloning and heterologous expression of a cDNA encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana

被引：125

作者：

Schwender, J ^{[1
]}

Müller, C ^{[1
]}

Zeidler, J ^{[1
]}

Lichlenthaler, HK ^{[1
]}

机构：

[1] Univ Karlsruhe, Inst Bot, D-76128 Karlsruhe, Germany

来源：

FEBS LETTERS | 1999年 / 455卷 / 1-2期

关键词：

1-deoxy-D-xylulose; 1-deoxy-D-xylulose 5-phosphate reductoisomerase; 1-deoxy-D-xylulose 5-phosphate pathway; isoprenoid biosynthesis; 2-C-methyl-D-erythritol;

D O I：

10.1016/S0014-5793(99)00849-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various plant isoprenoids are synthesized via the non-mevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP) by an enzyme which was recently cloned from Escherichia coli, In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) Ive cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin specifically inhibits this DXR enzyme activity. (C) 1999 Federation of European Biochemical Societies.

引用

收藏

页码：140 / 144

页数：5

相关论文

共 22 条

[1] 2-C-METHYL-ERYTHRITOL, A NEW BRANCHED ALDITOL FROM CONVOLVULUS-GLOMERATUS [J].

ANTHONSEN, T ;

HAGEN, S ;

KAZI, MA ;

SHAH, SW ;

TAGAR, S .

ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY, 1976, 30 (01) :91-93

[2] Terpenoid biosynthesis from 1-deoxy-D-xylulose in higher plants by intramolecular skeletal rearrangement [J].

Arigoni, D ;

Sagner, S ;

Latzel, C ;

Eisenreich, W ;

Bacher, A ;

Zenk, MH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (20) :10600-10605

[3] Dedicated roles of plastid transketolases during the early onset of isoprenoid biogenesis in pepper fruits [J].

Bouvier, F ;

d'Harlingue, A ;

Suire, C ;

Backhaus, RA ;

Camara, B .

PLANT PHYSIOLOGY, 1998, 117 (04) :1423-1431

[4]

BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025

[5] Distribution of the mevalonate and glyceraldehyde phosphate/pyruvate pathways for isoprenoid biosynthesis in unicellular algae and the cyanobacterium Synechocystis PCC 6714 [J].

Disch, A ;

Schwender, J ;

Müller, C ;

Lichtenthaler, HK ;

Rohmer, M .

BIOCHEMICAL JOURNAL, 1998, 333 :381-388

[6] 2-C-METHYL-D-ERYTHRITOL IN LEAVES OF LIRIODENDRON-TULIPIFERA [J].

DITTRICH, P ;

ANGYAL, SJ .

PHYTOCHEMISTRY, 1988, 27 (03) :935-935

[7] Cell-free conversion of 1-deoxy-D-xylulose 5-phosphate and 2-C-methyl-D-erythritol 4-phosphate into β-carotene in higher plants and its inhibition by fosmidomycin [J].

Fellermeier, M ;

Kis, K ;

Sagner, S ;

Maier, U ;

Bacher, A ;

Zenk, MH .

TETRAHEDRON LETTERS, 1999, 40 (14) :2743-2746

[8] Fosmidomycin, a specific inhibitor of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in the nonmevalonate pathway for terpenoid biosynthesis [J].

Kuzuyama, T ;

Shimizu, T ;

Takahashi, S ;

Seto, H .

TETRAHEDRON LETTERS, 1998, 39 (43) :7913-7916

[9] Direct formation of 2-C-methyl-D-erythritol 4-phosphate from 1-deoxy-D-xylulose 5-phosphate by 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a new enzyme in the non-mevalonate pathway to isopentenyl diphosphate [J].

Kuzuyama, T ;

Takahashi, S ;

Watanabe, H ;

Seto, H .

TETRAHEDRON LETTERS, 1998, 39 (25) :4509-4512

[10] A family of transketolases that directs isoprenoid biosynthesis via a mevalonate-independent pathway [J].

Lange, BM ;

Wildung, MR ;

McCaskill, D ;

Croteau, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2100-2104