Regulation of Ca-sensitive inactivation of a 1-type Ca2+ channel by specific domains of β subunits

被引:19
作者
Cens, T [1 ]
Restituito, S [1 ]
Charnet, P [1 ]
机构
[1] CRBM, CNRS, F-34293 Montpellier 5, France
关键词
voltage clamp; beta(2)/beta(1) chimera; heterologous expression; Xenopus oocyte;
D O I
10.1016/S0014-5793(99)00463-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ca2+ channel auxiliary beta subunits have been shown to modulate voltage-dependent inactivation of various types of Ca2+ channels. The beta(1) and beta(2) subunits, that are differentially expressed with the L-type alpha(1) Ca2+ channel subunit in heart, muscle and brain, can specifically modulate the Ca2+-dependent inactivation kinetics. Their expression in Xenopus oocytes with the alpha(1C) subunit leads, in both cases, to biphasic Ca2+ current decays, the second phase being markedly slowed by expression of the beta(2) subunit, Using a series of beta subunit deletion mutants and chimeric constructs of beta(1) and beta(2) subunits, we show that the inhibitory site located on the amino-terminal region of the beta(2a), subunit is the major element of this regulation. These results thus suggest that different splice variants of the beta(2) subunit can modulate, in a specific way, the Ca2+ entry through L-type Ca2+ channels in different brain or heart regions. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:17 / 22
页数:6
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