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Effects of methyl jasmonate and salicylic acid on cell growth and cryptotanshinone formation in Ti transformed Salvia miltiorrhiza cell suspension cultures
被引:27
作者:
Chen, H
[1
]
Chen, F
[1
]
机构:
[1] Univ Hong Kong, Dept Bot, Hong Kong, Peoples R China
关键词:
cryptotanshinone;
ibuprofen;
methyl jasmonate;
salicylic acid;
Salvia miltiorrhiza;
D O I:
10.1023/A:1005551911450
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
Exogenous methyl jasmonate (MJ) or salicylic acid (SA) when applied alone failed to induce cryptotanshinone (a phytoalexin) formation in Ti-transformed Salvia miltiorrhiza cell suspension cultures. However, when applied in combination with yeast elicitor, SA at 50-500 mu M enhanced the yeast elicitor-induced cryptotanshinone formation while MJ reduced the yeast elicitor-induced cryptotanshinone formation. Ibuprofen at 100 mu M did not inhibit the yeast elicitor-induced cryptotanshinone formation. DMSO was superior to ethanol as a solvent for introducing MJ to the cell cultures.
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页码:803 / 807
页数:5
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