Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding

被引：119

作者：

Podevin, Nancy ^{[1
]}

Davies, Howard V. ^{[2
]}

Hartung, Frank ^{[3
]}

Nogue, Fabien ^{[4
]}

Casacuberta, Josep M. ^{[5
]}

机构：

[1] EFSA, Parma, Italy

[2] James Hutton Inst, Dundee DD2 5DA, Scotland

[3] JKI, Fed Res Ctr Cultivated Plants, Inst Biosafety Plant Biotechnol, D-06484 Quedlinburg, Germany

[4] INRA AgroParisTech, IJPB, UMR 1318, INRA Ctr Versailles, F-78026 Versailles, France

[5] Ctr Res Agr Genom CSIC IRTA UAB UB, Barcelona 08193, Spain

来源：

TRENDS IN BIOTECHNOLOGY | 2013年 / 31卷 / 06期

关键词：

site-directed nuclease; zinc-finger nucleases (ZFN); transcription activator-like effector nucleases (TALEN); meganuclease; plant breeding; genetically modified organism (GMO); ZINC-FINGER NUCLEASES; DNA-BINDING SPECIFICITY; STRAND BREAK REPAIR; HOMING ENDONUCLEASES; HOMOLOGOUS RECOMBINATION; TARGETED MUTAGENESIS; GENOME MODIFICATIONS; END RESECTION; ARABIDOPSIS; EXPRESSION;

D O I：

10.1016/j.tibtech.2013.03.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conventional plant breeding exploits existing genetic variability and introduces new variability by mutagenesis. This has proven highly successful in securing food supplies for an ever-growing human population. The use of genetically modified plants is a complementary approach but all plant breeding techniques have limitations. Here, we discuss how the recent evolution of targeted mutagenesis and DNA insertion techniques based on tailor-made site-directed nucleases (SDNs) provides opportunities to overcome such limitations. Plant breeding companies are exploiting SDNs to develop a new generation of crops with new and improved traits. Nevertheless, some technical limitations as well as significant uncertainties on the regulatory status of SDNs may challenge their use for commercial plant breeding.

引用

页码：375 / 383

页数：9

共 81 条

[41] De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks [J].