A novel approach to DNA damage assessments: Measurement of the thymine glycol lesion

被引：13

作者：

Bailey, DT ^{[1
]}

DeFedericis, HCC ^{[1
]}

Greene, KF ^{[1
]}

Iijima, H ^{[1
]}

Budzinski, EE ^{[1
]}

Patrzyc, HB ^{[1
]}

Dawidzik, JB ^{[1
]}

Box, HC ^{[1
]}

机构：

[1] Roswell Pk Canc Inst, Buffalo, NY 14263 USA

来源：

RADIATION RESEARCH | 2006年 / 165卷 / 04期

关键词：

D O I：

10.1667/RR3534.1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A different approach to the measurement of DNA damage has been developed based on the fact that many lesions can be excised from DNA in the form of modified dinucleoside monophosphates. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used in conjunction with isotopically labeled internal standards to quantify the lesion. The method has several advantages, including high sensitivity for the detection of dinucleoside monophosphates. The method was applied to the measurement of the 5,6-dihydroxy-5,6-dihydrothymine (thymine glycol) lesion in the DNA of mouse fibroblast cells exposed in culture to various treatments including ionizing radiation, UVC light and buthionine sulfoximine (BSO), an inhibitor of glutathione synthesis. The application of the method to the measurement of other DNA lesions is discussed. (c) 2006 by Radiation Research Society.

引用

页码：438 / 444

页数：7

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