An architectural role of the Escherichia coli chromatin protein FIS in organising DNA

被引：123

作者：

Schneider, R

Lurz, R

Lüder, G

Tolksdorf, C

Travers, A

Muskhelishvili, G

机构：

[1] LMU Muenchen, Inst Genet & Mikrobiol, D-80638 Munich, Germany

[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[3] LMU Muenchen, Lehrstuhl Angew Phys, Sekt Phys, D-80799 Munich, Germany

[4] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

NUCLEIC ACIDS RESEARCH | 2001年 / 29卷 / 24期

关键词：

D O I：

10.1093/nar/29.24.5107

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Escherichia coli chromatin protein FIS modulates the topology of DNA in a growth phase-dependent manner. In this study we have investigated the global effect of FIS binding on DNA architecture in vitro. We show that in supercoiled DNA molecules FIS binds at multiple sites in a non-random fashion and increases DNA branching. This global DNA reshaping effect is independent of the helical phasing of FIS binding sites. We propose, in addition to the previously inferred stabilisation of tightly bent DNA microloops in the upstream regions of certain promoters, that FIS may perform the distinct architectural function of organising branched plectonemes in the E.coli nucleoid.

引用

收藏

页码：5107 / 5114

页数：8

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