Fecal tumor necrosis factor α, eosinophil cationic protein and IgE levels in infants with cow's milk allergy and gastrointestinal manifestations

被引:37
作者
Kapel, N
Matarazzo, P
Haouchine, D
Abiola, N
Guérin, S
Magne, D
Gobert, JG
Dupont, C
机构
[1] GH Pitie Salpetriere, Lab Coprolog Fonct, AP HP, F-75651 Paris 13, France
[2] Univ Paris 05, Lab Biol Anim & Parasitaire, UFR Sci Biolog & Pharmaceut, Paris, France
[3] Hop St Vincent de Paul, Serv Pediat, Paris, France
[4] Hop St Vincent de Paul, Serv Biochim, Paris, France
关键词
tumor necrosis factor (TNF)alpha; eosinophil cationic protein (ECP); IgE; cow's milk allergy; feces;
D O I
10.1515/CCLM.1999.004
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Infants with atopic eczema exhibit a specific fecal protein pattern after oral challenge with cow's milk, characterized by an increase in both eosinophil cationic protein (ECP) and tumor necrosis factor (TNF)alpha. The aim of our study was to determine the pattern of these proteins in allergic infants with intestinal manifestations. TNF alpha, ECP and immunoglobulin E (IgE) were measured in stools from 13 infants with intestinal symptoms and 10 healthy infants. The allergic infants underwent two stool collections, one before a cow's milk challenge and the other after the challenge, either at the onset of clinical manifestations (n=6) or 15 days after the challenge if no clinical manifestations occurred (n=7). Baseline TNF alpha, ECP and IgE levels were low in all infants. The concentration of TNF alpha increased after the challenge in infants positive to challenge (p<0.05) but not in those negative to challenge. ECP and IgE levels remained low after the challenge in all the allergic infants. These data confirm that fecal TNF alpha. and ECP levels indicate various reaction types of food allergy and that different immunologic disturbances lead to atopic eczema or intestinal symptoms during food allergy. Fecal protein pattern can thus be a useful tool in diagnosing food allergy in infants with intestinal manifestations.
引用
收藏
页码:29 / 32
页数:4
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