Molecular absorption spectrophotometry using a scanner:: Proposal of a didactic experiment for ascorbic acid determination

被引：27

作者：

Gomes, Marcos S. ^{[1
]}

Trevizan, Lilian C. ^{[1
]}

Nobrega, Joaquim A. ^{[1
]}

Kamogawa, Marcos Y. ^{[2
]}

机构：

[1] Univ Fed Sao Carlos, Dept Quim, BR-13560970 Sao Carlos, SP, Brazil

[2] Univ Sao Paulo, Dept Quim, Escola Super Agr Luiz de Queiroz, Piracicaba, SP, Brazil

来源：

QUIMICA NOVA | 2008年 / 31卷 / 06期

关键词：

spectrophotometry; scanner; ascorbic acid;

D O I：

10.1590/S0100-40422008000600050

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

Quantitative analysis of colored substances is generally performed using a spectrophotometer for detection of radiation. However, the combination of digital images and mathematical conversion of colors is an alternative for quantitative procedures based on colored chemical reactions. This work proposes a didactic experiment using a scanner for determination of ascorbic acid in medicines based on the formation of the Prussian blue complex. Results were in good agreement with a conventional spectrophotometer method and the procedure is attractive for instrumental analysis undergraduate courses.

引用

页码：1577 / 1581

页数：5

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