Bioaccumulation of copper by Trichoderma viride

被引:153
作者
Arland, P [1 ]
Isar, J [1 ]
Saran, S [1 ]
Saxena, RK [1 ]
机构
[1] Univ Delhi, Dept Microbiol, New Delhi 110021, India
关键词
bioaccumulation; heavy metal; copper; Trichoderma viride;
D O I
10.1016/j.biortech.2005.04.046
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Studies were carried out on interaction of Trichoderma viride with copper and reports bioaccumulation as a mechanism of copper tolerance during growth. There was a marked increase in the lag phase of the growth, which was concentration dependent. At a concentration of 100 mg/L Of CUCl2 center dot 2H(2)O, 81% Of Cu(II) were removed by 3.4 g/L of the biomass in 72 h. The process was temperature and pH dependent. The maximum copper bioaccumulation occurred at 30 degrees C, pH 5.0. Metabolic inhibitors such as sodium azide (NaN3) and 2,4-dinitrophenol (2,4-DNP) drastically reduced the extent of Cu(II) bioaccumulation. Electron microscopy and cell fractionation studies revealed that 70-80% of copper was present as a layer on the cell wall surface. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1018 / 1025
页数:8
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