Gas chromatography analysis of serum cholesterol synthesis and absorption markers used to predict the efficacy of simvastatin in patients with coronary heart disease

被引：35

作者：

Wu, Wen-Feng ^{[1
,3
]}

Wang, Qi-Hui ^{[2
]}

Zhang, Tao ^{[3
]}

Mi, Shu-Hua ^{[3
]}

Liu, Yang ^{[2
]}

Wang, Lv-Ya ^{[1
,3
]}

机构：

[1] Beijing Inst Heart Lung & Blood Vessel Dis, Beijing 100029, Peoples R China

[2] Beijing Ctr Phys & Chem Anal, Beijing 100089, Peoples R China

[3] Capital Med Univ, Dept Cardiol, Beijing Anzhen Hosp, Beijing 100029, Peoples R China

来源：

CLINICAL BIOCHEMISTRY | 2013年 / 46卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Coronary heart disease; Cholesterol; Markers; Absorption and synthesis; Gas chromatography; EVALUATING ROSUVASTATIN JUPITER; C-REACTIVE PROTEIN; PRIMARY PREVENTION; NONCHOLESTEROL STEROLS; CARDIOVASCULAR RISK; SURROGATE MARKERS; STATINS; JUSTIFICATION; PARTICIPANTS; METAANALYSIS;

D O I：

10.1016/j.clinbiochem.2013.04.003

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: We investigated the changes in cholesterol absorption and synthesis markers before and after simvastatin therapy in Chinese patients with coronary heart disease. Design and method: We developed a gas chromatography method to identify cholesterol synthesis and absorption markers and measured them in patients with coronary heart disease. We then tested their use in predicting the efficacy of simvastatin in lowering cholesterol. Serum samples from 45 patients and 38 healthy humans (controls) were analyzed in a gas chromatography-flame ionization detector. Results: Squalene and five non-cholesterol sterols-desmosterol and lathosterol (synthesis markers) and campesterol, stigmasterol, and sitosterol (absorption markers)-were detected. The recovery rates of the markers were 95-102%. After simvastatin treatment for four weeks, the total cholesterol and low-density lipoprotein cholesterol levels had significantly decreased from the baseline values (p < 0.05). The baseline lathosterol level was significantly higher in good responders than in poor responders (p < 0.05), and the stigmasterol level was significantly lower in good responders than in poor responders (p <0.05). Conclusions: This method should be suitable for the detection of serum squalene and non-cholesterol markers and can be used to predict the efficacy of simvastatin in patients with coronary heart disease. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：993 / 998

页数：6

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