Covalent attachment of hybridizable oligonucleotides to glass supports

被引:101
作者
Joos, B
Kuster, H
Cone, R
机构
[1] Division of Infectious Diseases, Department of Medicine, University Hospital, CH-8091, Zurich
关键词
D O I
10.1006/abio.1997.2017
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, rapid, and efficient method for the covalent binding of oligonucleotides to solid glass supports was developed. Glass slides were derivatized with aminophenyl or aminopropyl silanes and 5'-succinylated target oligonucleotides were attached by carbodiimide-mediated coupling. Approximately 40 to 50% of the applied target oligonucleotides covalently bound to the derivatized glass. Hybridizations with radioactively labeled oligonucleotide probes showed that up to 90% of the attached oligonucleotides were available for hybridization. This system can conveniently be applied for studies on hybridization and detection of nucleic acids. (C) 1997 Academic Press.
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页码:96 / 101
页数:6
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