The role of 2-methyl-6-phytylbenzoquinone methyltransferase in determining tocopherol composition in Synechocystis sp PCC6803

被引：68

作者：

Shintani, DK

Cheng, ZG

DellaPenna, D ^{[1
]}

机构：

[1] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[2] Univ Nevada, Dept Biochem MS200, Reno, NV 89557 USA

来源：

FEBS LETTERS | 2002年 / 511卷 / 1-3期

关键词：

tocopherol; vitamin E; methyltransferase; plastoquinone; Synechocystis;

D O I：

10.1016/S0014-5793(01)03223-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A putative 2-methyl-6-phytylbenzoquinone (MPBQ) methyltransferase gene, SLL0418, was identified from the Synechocystis PCC6803 genome based on its homology to previously characterized gamma-tocopherol methyltransferases. Genetic and biochemical evidence confirmed open reading frame (ORF) SLL0418 encodes a MPBQ methyltransferase. An SLL0418 partial knockout mutant accumulated beta-tocopherol with no effect in the overall tocopherol content of the cell. In vitro assays of the SLL0418 gene expressed in Escherichia coli showed the enzyme efficiently catalyzes methylation of ring carbon 3 of MPBQ. In addition, the enzyme also catalyzes the methylation of ring carbon 3 of 2-methyl-6-solanylbenzoquinol in the terminal step of plastoquinone biosynthesis. (C) 2002 Federation of European Biochemical Sociaties. Published by Elsevier Science B.V. All rights reserved.

引用

页码：1 / 5

页数：5