In situ determination of bacterial growth by multiple headspace extraction gas chromatography

被引：23

作者：

Chai, Xin-Sheng ^{[1
]}

Dong, Chunxu ^{[1
,3
]}

Deng, Yulin ^{[1
,2
]}

机构：

[1] Georgia Inst Technol, Inst Paper Sci & Technol, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA

[3] Dalian Univ Technol, Sch Chem Engn, State Key Lab Fine Chem, Dalian 116012, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 20期

关键词：

D O I：

10.1021/ac801537x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper demonstrated an in situ headspace gas chromatography (GC) technique for monitoring bacterial growth using a commercial GC system with a multiple headspace extraction (sampling) mode. The technique was based on measuring the carbon dioxide mass in the headspace of a closed sample vial during the bacteria growth. A mathematic equation was derived in order to calculate the integrated amount of carbon dioxide produced by bacterial growth during the incubation. The method can be used to monitor the bacterial growth rate in a given cultural medium. The present method is very simple, sensitive, and safe.

引用

页码：7820 / 7825

页数：6

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