Draw-fill batch culture mode for production of xylanases by Cellulomonas flavigena on sugar cane bagasse

被引：23

作者：

Vega-Estrada, J ^{[1
]}

Flores-Cotera, LB ^{[1
]}

Santiago, A ^{[1
]}

Magaña-Plaza, I ^{[1
]}

Montes-Horcasitas, C ^{[1
]}

机构：

[1] IPN, CINVESTAV, Biotechnol & Bioengn Dept, Mexico City 07360, DF, Mexico

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2002年 / 58卷 / 04期

关键词：

D O I：

10.1007/s00253-001-0908-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Draw-fill culture was evaluated as a method for xylanase production by Cellulomonas flavigena on sugar cane bagasse. Specific xylanase activity and volumetric xylanase activities were measured by harvesting 50%, 55%, 60% and 70% of fermented broth at the end of each subculture. Maximum specific (64 IU mg(-1) protein) and volumetric (166 IU ml(-1)) xylanase activities were obtained by harvesting 50-55% of broth. Values were 3.4 and 3.8 times greater than those obtained in batch cultures carried out under the same conditions. Enzyme productivity of 4.2 IU ml(-1) h(-1) was significantly greater than that obtained in continuous cultures (2.4 IU ml(-1) h(-1)) (P<0.05).

引用

页码：435 / 438

页数：4

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