The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells

被引：36

作者：

Karlsson, Goeran ^{[1
]}

Roerby, Emma ^{[2
]}

Pina, Cristina ^{[1
,4
]}

Soneji, Shamit ^{[1
,2
]}

Reckzeh, Kristian ^{[2
]}

Miharada, Kenichi ^{[2
]}

Karlsson, Christine ^{[2
]}

Guo, Yanping ^{[1
]}

Fugazza, Cristina ^{[1
]}

Gupta, Rajeev ^{[1
]}

Martens, Joost H. A. ^{[3
]}

Stunnenberg, Hendrik G. ^{[3
]}

Karlsson, Stefan ^{[2
]}

Enver, Tariq ^{[1
,2
]}

机构：

[1] UCL, Inst Canc, Stem Cell Lab, London WC1E 6BT, England

[2] Lund Univ, Lund Stem Cell Ctr, S-22184 Lund, Sweden

[3] Radboud Univ Nijmegen, Nijmegen Ctr Mol Life Sci, Dept Mol Biol, NL-6500 HB Nijmegen, Netherlands

[4] Univ Cambridge, Natl Hlth Serv Blood & Transplant, Dept Haematol, Cambridge CB2 0PT, England

来源：

CELL REPORTS | 2013年 / 4卷 / 04期

基金：

瑞典研究理事会;

关键词：

SELF-RENEWAL; LINEAGE COMMITMENT; PROGENITOR CELLS; IN-VIVO; EXPRESSION; IDENTIFICATION;

D O I：

10.1016/j.celrep.2013.07.020

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.

引用

页码：642 / 648

页数：7

共 18 条

[1]

Upregulation of flt3 expression within the bone marrow Lin-Sca1+c-kit+ stem cell compartment is accompanied by loss of self-renewal capacity [J].

Adolfsson, J ;

Borge, OJ ;

Bryder, D ;

Theilgaard-Mönch, K ;

Åstrand-Grundström, I ;

Sitnicka, E ;

Sasaki, Y ;

Jacobsen, SEW .

IMMUNITY, 2001, 15 (04) :659-669

[2]

Identification of Flt3+ lympho-myeloid stem cells lacking erythro-megakaryocytic potential:: A revised road map for adult blood lineage commitment [J].

Adolfsson, J ;

Månsson, R ;

Buza-Vidas, N ;

Hultquist, A ;

Liuba, K ;

Jensen, CT ;

Bryder, D ;

Yang, LP ;

Borge, OJ ;

Thoren, LAM ;

Anderson, K ;

Sitnicka, E ;

Sasaki, Y ;

Sigvardsson, M ;

Jacobsen, SEW .

CELL, 2005, 121 (02) :295-306

[3]

Molecular signatures of self-renewal, differentiation, and lineage choice in multipotential hemopoietic progenitor cells in vitro [J].

Bruno, L ;

Hoffmann, R ;

McBlane, F ;

Brown, J ;

Gupta, R ;

Joshi, C ;

Pearson, S ;

Seidl, T ;

Heyworth, C ;

Enver, T .

MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (02) :741-756

[4]

Distinct Hematopoietic Stem Cell Subtypes Are Differentially Regulated by TGF-β1 [J].

Challen, Grant A. ;

Boles, Nathan C. ;

Chambers, Stuart M. ;

Goodell, Margaret A. .

CELL STEM CELL, 2010, 6 (03) :265-278

[5]

In vivo proliferation and cell cycle kinetics of long-term self-renewing hematopoietic stem cells [J].

Cheshier, SP ;

Morrison, SJ ;

Liao, XS ;

Weissman, IL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :3120-3125

[6]

Hematopoietic Stem Cell Heterogeneity Takes Center Stage [J].

Copley, Michael R. ;

Beer, Philip A. ;

Eaves, Connie J. .

CELL STEM CELL, 2012, 10 (06) :690-697

[7]

Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo [J].

Goodell, MA ;

Brose, K ;

Paradis, G ;

Conner, AS ;

Mulligan, RC .

JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (04) :1797-1806

[8]

Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential [J].

Kent, David G. ;

Copley, Michael R. ;

Benz, Claudia ;

Woehrer, Stefan ;

Dykstra, Brad J. ;

Ma, Elaine ;

Cheyne, John ;

Zhao, Yongjun ;

Bowie, Michelle B. ;

Zhao, Yun ;

Gasparetto, Maura ;

Delaney, Allen ;

Smith, Clayton ;

Marra, Marco ;

Eaves, Connie J. .

BLOOD, 2009, 113 (25) :6342-6350

[9]

SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells [J].

Kiel, MJ ;

Yilmaz, ÖH ;

Iwashita, T ;

Yilmaz, OH ;

Terhorst, C ;

Morrison, SJ .

CELL, 2005, 121 (07) :1109-1121

[10]

Molecular evidence for hierarchical transcriptional lineage priming in fetal and adult stem cells and multipotent progenitors [J].

Mansson, Robert ;

Hultquist, Anne ;

Luc, Sidinh ;

Yang, Liping ;

Anderson, Kristina ;

Kharazi, Shabnam ;

Al-Hashmi, Suleiman ;

Liuba, Karina ;

Thoren, Lina ;

Adolfsson, Jorgen ;

Buza-Vidas, Natalija ;

Qian, Hong ;

Soneji, Shamit ;

Enver, Tariq ;

Sigvardsson, Mikael ;

Jacobsen, Sten Eirik W. .

IMMUNITY, 2007, 26 (04) :407-419

← 1 2 →