Deflazacort increases osteoclast formation in mouse bone marrow culture and the ratio of RANKL/OPG mRNA expression in marrow stromal cells

被引:11
作者
Chung, H
Kang, YS
Hwang, CS
Moon, IK
Yim, CH
Choi, KH
Han, KO
Jang, HC
Yoon, HK
Han, IK
机构
[1] Sungkyunkwan Univ, Dept Med Endocrinol,Lab Endocrinol, Sch Med,Samsung Cheil Hosp, Jung Ku, Seoul 100380, South Korea
[2] Sungkyunkwan Univ, Dept Obstet & Gynecol, Sch Med, Samsung Cheil Hosp, Seoul 100380, South Korea
关键词
glucocorticoids; osteoclasts; OPG; RANKL;
D O I
10.3346/jkms.2001.16.6.769
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Information on precise effects of deflazacort on bone cell function, especially osteoclasts, is quite limited. Therefore, the present study was undertaken to test effects of deflazacort on osteoclast-like cell formation in mouse bone marrow cultures and on the regulation of osteoprotegerin (OPG) and its ligand (RANKL) mRNA expressions by RT-PCR in the ST2 marrow stromal cells. TRAP-positive mononuclear cells increased after the treatment of deflazacort at 10(-9) to 10(-7) M alone for 6 days in a dose-dependent manner. Number of TRAP-positive multinucleated cells (MNCs) increased significantly with combined treatment of deflazacort at 10(-7) M and 1,25-(OH)(2)D-3 at 10(-9) M compared to that of cultures treated with 1,25-(OH)(2)D-3 alone (p <0.05). Exposure to deflazacort at 10(-7) M in the presence of 1,25-(OH)(2)D-3 at 10(-9) M in the last 3-day culture had greater stimulatory effect on osteoclast-like cell formation than that of the first 3-day culture did. Deflazacort at 10(-10)-10(-6) M downregulated OPG and upregulated RANKL in mRNA levels in a dose-dependent manner. These observations suggest that deflazacort stimulate osteoclast precursor in the absence of 1,25-(OH)(2)D-3 and enhance differentiation of osteoclasts in the presence of 1,25-(OH)(2)D-3. These effects are, in part, thought to be mediated by the regulation of the expression of OPG and RANKL mRNA in marrow stromal cells.
引用
收藏
页码:769 / 773
页数:5
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