LXR-Agonists Regulate ApoM Expression Differentially in Liver and Intestine

被引：28

作者：

Calayir, Emine ^{[1
]}

Becker, Tatjana M. ^{[1
]}

Kratzer, Adelheid ^{[1
]}

Ebner, Birgit ^{[1
]}

Panzenboeck, Ute ^{[2
]}

Stefulj, Jasminka ^{[2
]}

Kostner, Gerhard M. ^{[1
]}

机构：

[1] Med Univ Graz, Inst Mol Biol & Biochem, Ctr Mol Med, A-8010 Graz, Austria

[2] Med Univ Graz, Inst Pathophysiol & Immunol, Ctr Mol Med, A-8010 Graz, Austria

来源：

CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2008年 / 9卷 / 06期

关键词：

Intestine; lipid metabolism; lipocalin; expression profiling;

D O I：

10.2174/138920108786786376

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Apolipoprotein M ( apoM) has been suggested to play a role in reverse cholesterol transport. Here we studied the influence of liver X-receptor (LXR) agonist on the transcriptional regulation of apoM. Studies were performed in murine liver and intestinal mucosal cells in vivo and in human intestinal Caco-2 cells in vitro. The expression of apoM was analyzed by quantitative real time PCR, and compared to well-established LXR target genes. Mice fed with TO901317 for six days showed a downregulation of apoM and apoAI in the liver to 40 % and 60 % respectively and an upregulation of Cyp7A1 to 280 %. In the small intestine, however, apoM and apoAI were upregulated by 30-60 % and ABCA1 by 250-430 %. In Caco-2 cells TO901317 caused a 60 % upregulation and the natural LXR agonist 22-hydroxycholesterol a 40 % upregulation of apoM. Possible causes for the differential effects in liver and intestine are discussed.

引用

页码：516 / 521

页数：6

共 15 条

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