Application of four Elisa techniques (two for IgM and two for IgG) for serological diagnosis of an outbreak of Q fever

被引：11

作者：

Carlos Sanz, Juan

de los Rios, Rosa

Martin, Fernando

Angeles Tebar, M.

Jado, Isabel

Anda, Pedro

机构：

[1] Inst Salud Publ, Lab Reg Salud Publ, Madrid 28006, Spain

[2] Comunidad Madrid, Inst Salud Publ, Serv Salud Publ Area 4, Madrid, Spain

[3] Inst Salud Carlos III, Ctr Nacl Microbiol, Madrid, Spain

来源：

ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2006年 / 24卷 / 03期

关键词：

Q fever; serology; ELISA; outbreak;

D O I：

10.1157/13086551

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

OBJECTIVE. To evaluate four ELISAs (commercialized by Panbio and Serion) for IgM and IgG detection in an outbreak of 0 fever. METHODS. Fifty-three serum samples corresponding to 29 persons were processed. An indirect immunofluorescence assay was used as the reference method. RESULTS. The sensitivity and specificity for IgM were 100% and 74% (Panbio), and 91% and 88% (Serion). IgG sensitivity was 72% (Panbio) and 89% (Serion). The specificity of both techniques for IgG was 97%. CONCLUSIONS. Both ELISAs seem to be good IgM screening methods for use during Q fever outbreaks.

引用

页码：178 / 181

页数：4