A ligand-gated, hinged loop rearrangement opens a channel to a buried artificial protein cavity

被引：47

作者：

Fitzgerald, MM ^{[1
]}

Musah, RA ^{[1
]}

McRee, DE ^{[1
]}

Goodin, DB ^{[1
]}

机构：

[1] SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1996年 / 3卷 / 07期

关键词：

D O I：

10.1038/nsb0796-626

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conformational changes that gate the access of substrates or ligands to an active site are important features of enzyme function. In this report, we describe an unusual example of a structural rearrangement near a buried artificial cavity in cytochrome c peroxidase that occurs on binding protonated benzimidazole. A hinged main-chain rotation at two residues (Pro 190 and Asn 195) results in a surface loop rearrangement that opens a large solvent-accessible channel for the entry of ligands to an otherwise inaccessible binding site. The trapping of this alternate conformational state provides a unique view of the extent to which protein dynamics can allow small molecule penetration into buried protein cavities.

引用

页码：626 / 631

页数：6

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