Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/beta ig-h3) that binds to collagen

被引:144
作者
Hashimoto, K
Noshiro, M
Ohno, S
Kawamoto, T
Satakeda, H
Akagawa, Y
Nakashima, K
Okimura, A
Ishida, H
Okamoto, T
Pan, H
Shen, M
Yan, WQ
Kato, Y
机构
[1] HIROSHIMA UNIV,SCH DENT,DEPT BIOCHEM,MINAMI KU,HIROSHIMA 734,JAPAN
[2] HIROSHIMA UNIV,SCH DENT,DEPT REMOVAL PROSTHODONT,MINAMI KU,HIROSHIMA 734,JAPAN
[3] HIROSHIMA UNIV,SCH DENT,DEPT ORTHODONT,HIROSHIMA 734,JAPAN
[4] HIROSHIMA UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG 1,MINAMI KU,HIROSHIMA 734,JAPAN
[5] NIPPON HOECHST MARION ROUSSEL,LAB BONE RES,KAWAGOE,SAITAMA 350,JAPAN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1997年 / 1355卷 / 03期
关键词
collagen-binding protein; cartilage; chondrocyte; collagen; RGD; TGF-beta;
D O I
10.1016/S0167-4889(96)00147-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 66-kDa collagen fiber-associated protein (RGD-CAP) was isolated from a fiber-rich fraction of pig cartilage by ultrafiltration and collagen-affinity chromatography. Amino acid sequencing and cDNA cloning indicated that the RGD-CAP is identical or closely related to beta ig-h3 protein which is induced in human adenocarcinoma cells by transforming growth factor-beta (TGF-beta) (Skonier, J., Neubauer, M., Madisen, L., Bennett, K., Plowman, G.D., and Purchio, A.F. (1992) DNA Cell. Biol. 11, 511-522). The RGD-CAP, as well as beta ig-h3, has the RGD sequence in the C-terminal region. The native RGD-CAP bound to type I, II, and IV collagens even in the presence of 1 M NaCl. A recombinant preparation of RGD-CAP expressed in Escherichia coli cells also bound to collagen but not to gelatin. The RGD-CAP mRNA was expressed in chondrocytes throughout all stages, although the expression level was highest during the prehypertrophic stage. In addition, TGF-beta increased the RGD-CAP mRNA level in chondrocyte cultures. Since RGD-CAP transcripts were found in most tissues, this novel collagen-binding protein may play an important role in cell-collagen interactions in various tissues including developing cartilage.
引用
收藏
页码:303 / 314
页数:12
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