The "tale" of UmuD and its role in SOS mutagenesis

被引:28
作者
Gonzalez, M
Woodgate, R
机构
[1] Univ Colorado, Dept Biol, Denver, CO 80202 USA
[2] NICHHD, Sect DNA Replicat Repair & Mutagenesis, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1002/bies.10040
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recently, the Escherichia coli umuD and umuC genew have been shown to encode E. coli's fifth DNA polymerase, pol V (consisting of a heterotrimer of UmuD'C-2). The main function of pol V appears to be the bypass of DNA lesions that would otherwise block replication by pols I-IV. This process is error-prone and leads to a striking increase in mutations at sites of DNA damage. While the enzymatic properties of pol V are now only beginning to be fully appreciated, a great deal is known about how E. coli regulates the intracellular levels of the Umu proteins so that the lesion-bypassing activity of pol V is available to help cells survive the deleterious consequences of DNA damage, yet keeps any unwarranted activity on undamaged templates to a minimum. Our review summarizes the multiple restrictions imposed upon pol V, so as to limit its activity in vivo and, in particular, highlights the pivotal role that the N-terminal tail of UmuD plays in regulating SOS mutagenesis. BioEssays 24:1,41-148, 2002. Published 2002 Wiley Periodicals, Inc.(dagger).
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收藏
页码:141 / 148
页数:8
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