Genome-wide analysis of spatial and temporal gene expression in rice panicle development

被引:90
作者
Furutani, I
Sukegawa, S
Kyozuka, J
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Tokyo 1130032, Japan
[2] Japan Sci & Technol Corp, CREST, Kawaguchi, Saitama 3320012, Japan
关键词
shoot branching; inflorescence; rice panicle; microarray; LAX;
D O I
10.1111/j.1365-313X.2006.02703.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The basic structure of a rice inflorescence (the panicle) is determined by the pattern of branch formation, which is established at the early stages of panicle development. In this study we conducted global transcriptome profiling of the early stages of rice panicle development from phase transition to floral organ differentiation. To generate a meristem-specific gene-expression profile, shoot apical meristems (SAMs) and subsequently formed, very young panicles were collected manually and used for cDNA microarray analysis. We identified 357 out of 22 000 genes that are expressed differentially in the early stages of panicle development, and the 357 genes were classified into seven groups based on their temporal expression patterns. The most noticeable feature is that a fairly small number of genes, which are extensively enriched in transcription factors, are upregulated in the SAM immediately after phase transition. In situ hybridization analysis showed that each gene analysed exhibits a unique and interesting localization of mRNA. Remarkably, one of the transcription factors was proven to be a close downstream component of the pathway in which LAX, a major regulator of panicle branching, acts. These results suggest that our strategy - careful collection of meristems, global transcriptome analysis and subsequent in situ hybridization analysis - is useful not only to obtain a genome-wide view of gene expression, but also to reveal genetic networks controlling rice panicle development.
引用
收藏
页码:503 / 511
页数:9
相关论文
共 39 条
[1]   FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex [J].
Abe, M ;
Kobayashi, Y ;
Yamamoto, S ;
Daimon, Y ;
Yamaguchi, A ;
Ikeda, Y ;
Ichinoki, H ;
Notaguchi, M ;
Goto, K ;
Araki, T .
SCIENCE, 2005, 309 (5737) :1052-1056
[2]   Genes involved in organ separation in Arabidopsis: An analysis of the cup-shaped cotyledon mutant [J].
Aida, M ;
Ishida, T ;
Fukaki, H ;
Fujisawa, H ;
Tasaka, M .
PLANT CELL, 1997, 9 (06) :841-857
[3]   A gene expression map of the Arabidopsis root [J].
Birnbaum, K ;
Shasha, DE ;
Wang, JY ;
Jung, JW ;
Lambert, GM ;
Galbraith, DW ;
Benfey, PN .
SCIENCE, 2003, 302 (5652) :1956-1960
[4]   Genetics and evolution of inflorescence and flower development in grasses [J].
Bommert, P ;
Satoh-Nagasawa, N ;
Jackson, D ;
Hirano, HY .
PLANT AND CELL PHYSIOLOGY, 2005, 46 (01) :69-78
[5]   The control of spikelet meristem identity by the branched silkless1 gene in maize [J].
Chuck, G ;
Muszynski, M ;
Kellogg, E ;
Hake, S ;
Schmidt, RJ .
SCIENCE, 2002, 298 (5596) :1238-1241
[6]   The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity [J].
Ditta, G ;
Pinyopich, A ;
Robles, P ;
Pelaz, S ;
Yanofsky, MF .
CURRENT BIOLOGY, 2004, 14 (21) :1935-1940
[7]   Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT-Iike gene expression independently of Hd1l [J].
Doi, K ;
Izawa, T ;
Fuse, T ;
Yamanouchi, U ;
Kubo, T ;
Shimatani, Z ;
Yano, M ;
Yoshimura, A .
GENES & DEVELOPMENT, 2004, 18 (08) :926-936
[8]  
Ferrándiz C, 2000, DEVELOPMENT, V127, P725
[9]   The role of barren stalk1 in the architecture of maize [J].
Gallavotti, A ;
Zhao, Q ;
Kyozuka, J ;
Meeley, RB ;
Ritter, M ;
Doebley, JF ;
Pè, ME ;
Schmidt, RJ .
NATURE, 2004, 432 (7017) :630-635
[10]   Molecular analysis of the LATERAL SUPPRESSOR gene in Arabidopsis reveals a conserved control mechanism for axillary meristem formation [J].
Greb, T ;
Clarenz, O ;
Schäfer, E ;
Müller, D ;
Herrero, R ;
Schmitz, G ;
Theres, K .
GENES & DEVELOPMENT, 2003, 17 (09) :1175-1187