Endothelin-1 myocardial clearance, production, and effect on capillary permeability in vivo

被引:18
作者
Dupuis, J
Goresky, CA
Rose, CP
Stewart, DJ
Cernacek, P
Schwab, AJ
Simard, A
机构
[1] Montreal Heart Inst, Res Ctr, Dept Med, Montreal, PQ H1T 1C8, CANADA
[2] Montreal Gen Hosp, Dept Med, Montreal, PQ H3G 1A4, CANADA
[3] Royal Victoria Hosp, Dept Med, Montreal, PQ H3A 1A1, CANADA
[4] St Michaels Hosp, Dept Med, Toronto, ON M5B 1W8, CANADA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1997年 / 273卷 / 03期
关键词
coronary flow; indicator-dilution technique;
D O I
10.1152/ajpheart.1997.273.3.H1239
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Myocardial metabolism of endothelin-1 (ET-1) and its effect on coronary microcirculatory exhanges were obtained in anesthetized dogs by combining the indicator-dilution technique with immunoreactive ET-1 measurements. The myocardium extracted 17.7 +/- 4.6% of tracer ET-1 (n = 12). Simultaneously measured ET-1 levels in the aorta (0.97 +/- 0.46 pg/ml) and coronary sinus (0.96 +/- 0.53 pg/ml) were not different, supporting a production of ET-1 by the heart that balances the amount extracted. Intracoronary infusion of ET-1 (5 ng . kg(-1) . min(-1)) increased coronary sinus ET-1 levels similar to 50-fold, decreased coronary blood flow per unit of interstitial space by similar to 30% (P = 0.006), and increased myocardial microcirculatory transit times (n = 6). Permeability to albumin was unaffected by ET-1, whereas the permeability-surface area product for sucrose decreased following derecruitment of myocardial capillaries. We conclude that there is a normal myocardial metabolic balance of ET-1 and that the heart marginally contributes to circulating ET-1. Pharmacological doses of ET-1 may adversely affect myocardial metabolism by reducing blood flow and the permeability-surface area product for small circulating substances.
引用
收藏
页码:H1239 / H1245
页数:7
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