The cystic fibrosis transmembrane conductance regulator (CFTR) inhibits ENaC through an increase in the intracellular Cl- concentration

被引：80

作者：

König, J

Schreiber, R

Voelcker, T

Mall, M

Kunzelmann, K ^{[1
]}

机构：

[1] Univ Queensland, Dept Physiol & Pharmacol, Sch Biomed Sci, St Lucia, Qld 4072, Australia

[2] Univ Freiburg, Kinderklin, D-79106 Freiburg, Germany

来源：

EMBO REPORTS | 2001年 / 2卷 / 11期

关键词：

D O I：

10.1093/embo-reports/kve232

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activation of the CFTR Cl- channel inhibits epithelial Na+ channels (ENaC), according to studies on epithelial cells and overexpressing recombinant cells. Here we demonstrate that ENaC is inhibited during stimulation of the cystic fibrosis trans-membrane conductance regulator (CFTR) in Xenopus oocytes, independent of the experimental set-up and the magnitude of the whole-cell current. Inhibition of ENaC is augmented at higher CFTR Cl- currents. Similar to CFTR, ClC-0 Cl- currents also inhibit ENaC, as well as high extracellular Na+ and Cl- in partially permeabilized oocytes. Thus, inhibition of ENaC is not specific to CFTR and seems to be mediated by Cl-.

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页码：1047 / 1051

页数：5

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