Generation of E3-deleted canine adenoviruses expressing canine parvovirus capsid by homologous recombination in bacteria

被引：15

作者：

Morrison, MD

Reid, D

Onions, D

Spibey, N

Nicolson, L

机构：

[1] Univ Glasgow, Sch Vet, Dept Vet Pathol, Glasgow G61 1QH, Lanark, Scotland

[2] QOne Biotech Ltd, Glasgow G20 0XA, Lanark, Scotland

[3] Intervet UK Ltd, Houghton PE17 2BQ, Cambs, England

来源：

VIROLOGY | 2002年 / 293卷 / 01期

关键词：

CAV-1; BJ5183; cells; vaccine;

D O I：

10.1006/viro.2001.1262

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

E3-deleted canine adenovirus type 1 (CAV-1) was generated by homologous recombination in bacterial cells, using an antibiotic resistance marker to facilitate the recovery of recombinants. This marker was flanked by unique restriction endonuclease sites, which allowed its subsequent removal and the insertion of cassettes expressing the canine parvovirus capsid at the E3 locus. Infectious virus was recovered following transfection of canine cells and capsid expression was observed by RT-PCR from one of the virus constructs. A second construct, containing a different promoter, showed delayed growth and genome instability which, based on the size difference between these inserts, suggests a maximum packaging size of 106 to 109% wild-type genome size for CAV-1. (C) 2002 Elsevier Science (USA).

引用

页码：26 / 30

页数：5

共 16 条

[1] IMPROVED ANTIBIOTIC-RESISTANCE GENE CASSETTES AND OMEGA-ELEMENTS FOR ESCHERICHIA-COLI VECTOR CONSTRUCTION AND IN-VITRO DELETION INSERTION MUTAGENESIS [J].