IMPROVED ANTIBIOTIC-RESISTANCE GENE CASSETTES AND OMEGA-ELEMENTS FOR ESCHERICHIA-COLI VECTOR CONSTRUCTION AND IN-VITRO DELETION INSERTION MUTAGENESIS

被引：156

作者：

ALEXEYEV, MF

SHOKOLENKO, IN

CROUGHAN, TP

机构：

[1] LOUISIANA STATE UNIV,CTR AGR,LOUISIANA AGR EXPT STN,RICE RES STN,CROWLEY,LA 70527

[2] NATL ACAD SCI UKRAINE,INST MOLEC BIOL & GENET,KIEV 252143,UKRAINE

来源：

GENE | 1995年 / 160卷 / 01期

关键词：

CHLORAMPHENICOL ACETYLTRANSFERASE; BETA-LACTAMASE; NEOMYCIN PHOSPHOTRANSFERASE; GENTAMICIN ACETYLTRANSFERASE-3-1; STREPTOMYCIN-RESISTANCE GENE; TETRACYCLINE-RESISTANCE GENE;

D O I：

10.1016/0378-1119(95)00108-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Several antibiotic-resistance gene cassettes and Omega elements for Escherichia coli vector construction include the aacC1, aadA(+), bla, cat, nptII and tet gene cassettes, and also the Omega-Gm, Omega-Sm, Omega-Ap, Omega-Cm, Omega-Km and Omega-Tc elements. Both cassettes and elements are flanked by pBluescriptII plasmid multiple cloning sites (MCS) duplicated in inverted (symmetric MCS) or direct (tandem MCS) orientation, Genes that were modified in order to remove sites for the most common restriction endonucleases from their coding regions (except aacC1 and aadA(+)) were used for cassette and Omega-element construction.

引用

页码：63 / 67

页数：5

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