Down-regulation of miRNA-221 triggers osteogenic differentiation in human stem cells

被引：60

作者：

Bakhshandeh, Behnaz ^{[2
,3
]}

Hafizi, Maryam ^{[3
]}

Ghaemi, Nasser ^{[4
]}

Soleimani, Masoud ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Dept Hematol, Fac Med Sci, Tehran, Iran

[2] Univ Tehran, Dept Biotechnol, Coll Sci, Tehran, Iran

[3] Stem Cell Technol Res Ctr, Stem Cell Biol Dept, Tehran, Iran

[4] Univ Tehran, Sch Chem, Coll Sci, Tehran, Iran

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 08期

关键词：

Ectopic transfection; Mesenchymal stem cells; microRNA-221; Osteogenic differentiation; Unrestricted somatic stem cells; CORD BLOOD; BETA; WNT;

D O I：

10.1007/s10529-012-0934-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Despite interesting in silico evidence, the specific role of mir-221 in osteogenesis has not been studied. We evaluated the osteogenic induction of transient-transfected anti-mir-221 in human unrestricted somatic stem cells and human mesenchymal stem cells both transcriptionally and translationally. In transfected unrestricted somatic stem cells, transcriptions of some osteogenic markers were twice that of the control and translations of osteopontin and osteocalcin were increased from 9 to 39 % and from 0 to 21 %, respectively. Up-regulation of transcribed osteogenic markers in transfected mesenchymal stem cells were 50 times greater than controls while no significant change in translations were observed. Prior to these analyses, the authenticity of stem cells, their osteogenic differentiation and transfection efficiency were verified. Transient modulation of mir-221 therefore suggests a mechanism for rapid induction of osteogenesis as a useful strategy for cell-based therapy.

引用

页码：1579 / 1587

页数：9

共 33 条

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