NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate

被引：66

作者：

Bera, TK ^{[1
]}

Das, S ^{[1
]}

Maeda, H ^{[1
]}

Beers, R ^{[1
]}

Wolfgang, CD ^{[1
]}

Kumar, V ^{[1
]}

Hahn, Y ^{[1
]}

Lee, BK ^{[1
]}

Pastan, I ^{[1
]}

机构：

[1] NCI, Mol Biol Lab, Canc Res Ctr, NIH, Bethesda, MD 20892 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 09期

关键词：

D O I：

10.1073/pnas.0308746101

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We identified a gene (NGEP) that is expressed only in prostate cancer and normal prostate. The two NGEP transcripts are 0.9 kb and 3.5 kb in size and are generated by a differential splicing event. The short variant (NGEP-S) is derived from four exons and encodes a 20-kDa intracellular protein. The long form (NGEP-L) is derived from 18 exons and encodes a 95-kDa protein that is predicted to contain seven-membrane-spanning regions. In situ hybridization shows that NGEP mRNA is localized in epithelia[ cells of normal prostate and prostate cancers. Immunocytochemical analysis of cells transfected with NGEP cDNAs containing a Myc epitope tag at the carboxyl terminus shows that the protein encoded by the short transcript is localized in the cytoplasm, whereas the protein encoded by the long transcript is present on the plasma membrane. Because of its selective expression in prostate cancer and its presence on the cell surface, NGEP-L is a promising target for the antibody-based therapies of prostate cancer.

引用

页码：3059 / 3064

页数：6

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