Capillary electrochromatography of peptides in a microfabricated system

被引：77

作者：

He, B ^{[1
]}

Ji, JY ^{[1
]}

Regnier, FE ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 853卷 / 1-2期

关键词：

electrochromatography; capillary columns; peptides;

D O I：

10.1016/S0021-9673(99)00572-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversed-phase liquid chromatography of tryptic peptides is shown in the capillary electrochromatography mode using microfabricated columns. Although selectivity is different, a mixture of tryptic peptides from ovalbumin appears to be as easily separated in the CEC as HPLC mode. The major difference between a separation in the microfabricated CEC column and conventional separations in the HPLC mode is that separations are more readily achieved in the isocratic mode in the lower surface area microfabricated CEC columns. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：257 / 262

页数：6

共 17 条

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