miR-26a Suppresses Tumor Growth and Metastasis by Targeting FGF9 in Gastric Cancer

被引:89
作者
Deng, Min [1 ,2 ,4 ]
Tang, Hai-lin [3 ,4 ]
Lu, Xi-hong [5 ]
Liu, Mei-yuan [1 ,2 ]
Lu, Xiao-min
Gu, Yi-xue [1 ,2 ]
Liu, Ji-fang [1 ,2 ,4 ]
He, Zhi-min [1 ,2 ]
机构
[1] Guangzhou Med Univ, Canc Hosp, Guangzhou, Guangdong, Peoples R China
[2] Guangzhou Med Univ, Canc Res Inst, Guangzhou, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Ctr Canc, Dept Breast Oncol, Guangzhou 510275, Guangdong, Peoples R China
[4] Univ South China, Canc Res Inst, Hengyang, Peoples R China
[5] Univ South China, Affiliated Nanhua Hosp, Hengyang, Peoples R China
关键词
NASOPHARYNGEAL CARCINOMA; MICRORNA EXPRESSION; SURVIVAL; CLUSTER; GLIOMA; CELLS; EZH2;
D O I
10.1371/journal.pone.0072662
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
The role of miR-26a in cancer cells seemed controversial in previous studies. Until now, the role of miR-26a in gastric cancer remains undefined. In this study, we found that miR-26a was strongly downregulated in gastric cancer (GC) tissues and cell lines, and its expression levels were associated with lymph node metastasis and clinical stage, as well as overall survival and replase-free survival of GC. We also found that ectopic expression of miR-26a inhibited GC cell proliferation and GC metastasis in vitro and in vivo. We further identified a novel mechanism of miR-26a to suppress GC growth and metastasis. FGF9 was proved to be a direct target of miR-26a, using luciferase assay and western blot. FGF9 overexpression in miR-26a-expressing cells could rescue invasion and growth defects of miR-26a. In addition, miR-26a expression inversely correlated with FGF9 protein levels in GC. Taken together, our data suggest that miR-26a functions as a tumor suppressor in GC development and progression, and holds promise as a prognostic biomarker and potential therapeutic target for GC.
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页数:10
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