The interferon-inducible nucleolar p204 protein binds the ribosomal RNA-specific UBF1 transcription factor and inhibits ribosomal RNA transcription

被引:69
作者
Liu, CJ [1 ]
Wang, H [1 ]
Lengyel, P [1 ]
机构
[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
关键词
interferon; p204; rRNA; transcription; UBF;
D O I
10.1093/emboj/18.10.2845
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
p204, a member of the interferon-inducible p200 family of murine proteins, is primarily nucleolar, We generated cell lines in which p204 was inducible by muristerone, This induction resulted in retardation of cell proliferation and inhibition of rRNA transcription in vivo. Interferon treatment, resulting in p204 induction and retardation of proliferation, also caused inhibition of rRNA transcription in vivo. p204 also inhibited rRNA transcription in vitro. This inhibition was overcome by addition of UBF1, the rRNA-specific transcription factor. A direct interaction between p204 and UBF1 was revealed in vitro in pull-down assays, and in vivo by co-immunoprecipitation from cell extracts. UBF1 bound strongly to at least two regions of p204: the N-terminal segment linked to the conserved 200 amino acid a segment, and the conserved 200 amino acid b segment. Cleavage of the a or b segments into two segments (encoded by single exons) resulted in a strong decrease or loss of binding. The inhibition of rRNA transcription by p204 may be due to the inhibition by p204 of the specific DNA binding of UBF1. This was revealed in electrophoretic mobility shift, magnetic bead and footprinting assays. Thus, p204 serves as a mediator of the inhibition of rRNA transcription by interferon.
引用
收藏
页码:2845 / 2854
页数:10
相关论文
共 72 条
  • [31] Hempel WM, 1996, MOL CELL BIOL, V16, P557
  • [32] The retinoblastoma protein: A master regulator of cell cycle, differentiation and apoptosis
    Herwig, S
    Strauss, M
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 246 (03): : 581 - 601
  • [33] CLONING AND STRUCTURAL-ANALYSIS OF CDNA AND THE GENE FOR MOUSE TRANSCRIPTION FACTOR UBF
    HISATAKE, K
    NISHIMURA, T
    MAEDA, Y
    HANADA, K
    SONG, CZ
    MURAMATSU, M
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (17) : 4631 - 4637
  • [34] MULTIPLE DOMAINS OF THE RNA POLYMERASE-I ACTIVATOR HUBF INTERACT WITH THE TATA-BINDING PROTEIN COMPLEX HSL1 TO MEDIATE TRANSCRIPTION
    JANTZEN, HM
    CHOW, AM
    KING, DS
    TJIAN, R
    [J]. GENES & DEVELOPMENT, 1992, 6 (10) : 1950 - 1963
  • [35] NUCLEOLAR TRANSCRIPTION FACTOR HUBF CONTAINS A DNA-BINDING MOTIF WITH HOMOLOGY TO HMG PROTEINS
    JANTZEN, HM
    ADMON, A
    BELL, SP
    TJIAN, R
    [J]. NATURE, 1990, 344 (6269) : 830 - 836
  • [36] The human interferon-inducible protein, IFI 16, is a repressor of transcription
    Johnstone, RW
    Kerry, JA
    Trapani, JA
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (27) : 17172 - 17177
  • [37] Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein
    Kihm, AJ
    Hershey, JC
    Haystead, TAJ
    Madsen, CS
    Owens, GK
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) : 14816 - 14820
  • [38] p202 prevents apoptosis in murine AKR-2B fibroblasts
    Koul, D
    Lapushin, R
    Xu, HJ
    Mills, GB
    Gutterman, JU
    Choubey, D
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 247 (02) : 379 - 382
  • [39] FUNCTIONAL DIFFERENCES BETWEEN THE 2 SPLICE VARIANTS OF THE NUCLEOLAR TRANSCRIPTION FACTOR UBF - THE 2ND HMG BOX DETERMINES SPECIFICITY OF DNA-BINDING AND TRANSCRIPTIONAL ACTIVITY
    KUHN, A
    VOIT, R
    STEFANOVSKY, V
    EVERS, R
    BIANCHI, M
    GRUMMT, I
    [J]. EMBO JOURNAL, 1994, 13 (02) : 416 - 424
  • [40] DUAL ROLE OF THE NUCLEOLAR TRANSCRIPTION FACTOR UBF - TRANSACTIVATOR AND ANTIREPRESSOR
    KUHN, A
    GRUMMT, I
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) : 7340 - 7344