Conversion from CUL4-based COP1-SPA E3 apparatus to UVR8-COP1-SPA complexes underlies a distinct biochemical function of COP1 under UV-B

被引：159

作者：

Huang, Xi ^{[1
,2
]}

Ouyang, Xinhao ^{[1
,2
,3
]}

Yang, Panyu ^{[1
,4
]}

Lau, On Sun ^{[2
]}

Chen, Liangbi ^{[4
]}

Wei, Ning ^{[2
]}

Deng, Xing Wang ^{[1
,2
]}

机构：

[1] Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Beijing 100871, Peoples R China

[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

[3] Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Sichuan, Peoples R China

[4] Hunan Normal Univ, Coll Life Sci, Dept Bot, Changsha 410081, Hunan, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 41期

基金：

中国博士后科学基金;

关键词：

light signaling; protein complex; posttranscriptional regulation; CONSTITUTIVELY PHOTOMORPHOGENIC1; LIGHT CONTROL; ARABIDOPSIS; PROTEINS; HY5; TRANSCRIPTION; SUPPRESSION; PERCEPTION; INTERACTS; GROWTH;

D O I：

10.1073/pnas.1316622110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The evolutionarily conserved CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) is a RING and WD40 protein that functions as a substrate receptor of CULLIN4-DAMAGED DNA BINDING PROTEIN 1 (CUL4-DDB1)-based E3 ubiquitin ligases in both plants and animals. In Arabidopsis, COP1 is a central repressor of photomorphogenesis in the form of COP1-SUPPRESSOR OF PHYA (SPA) complex(es). CUL4-DDB1-COP1-SPA suppresses the photomorphogenic program by targeting the transcription factor ELONGATED HYPOCOTYL 5 for degradation. Intriguingly, under photomorphogenic UV-B light, COP1 reverses its repressive role and promotes photomorphogenesis. However, the mechanism by which COP1 is functionally switched is still obscure. Here, we demonstrate that UV-B triggers the physical and functional disassociation of the COP1-SPA core complex(es) from CUL4-DDB1 and the formation of a unique complex(es) containing the UV-B receptor UV RESISTANCE LOCUS 8 (UVR8). The establishment of this UV-B-dependent COP1 complex(es) is associated with its positive modulation of ELONGATED HYPOCOTYL 5 stability and activity, which sheds light on the mechanism of COP1's promotive action in UV-B-induced photomorphogenesis.

引用

页码：16669 / 16674

页数：6

共 27 条

[21] The COP1-SPA1 interaction defines a critical step in phytochrome A-mediated regulation of HY5 activity [J].