Development of mesenchymal stem cells partially originate from the neural crest

被引：196

作者：

Morikawa, Satoru ^{[1
,2
]}

Mabuchi, Yo ^{[1
]}

Niibe, Kunimichi ^{[1
,2
]}

Suzuki, Sadafumi ^{[1
]}

Nagoshi, Narihito ^{[1
,3
]}

Sunabori, Takehiko ^{[1
]}

Shimmura, Shigeto ^{[4
]}

Nagai, Yasuo ^{[1
]}

Nakagawa, Taneaki ^{[2
]}

Okano, Hideyuki ^{[1
]}

Matsuzaki, Yumi ^{[1
]}

机构：

[1] Keio Univ, Dept Physiol, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[2] Keio Univ, Dept Dent & Oral Surg, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[3] Keio Univ, Dept Orthoped Surg, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[4] Keio Univ, Dept Ophthalmol, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 04期

关键词：

Mesenchymal stem cells; Neural crest; Flow cytometry; Prospective isolation; Developmental origin; PROGENITOR CELLS; BONE-MARROW; NERVOUS-SYSTEM; GROWTH; DIFFERENTIATION; IDENTIFICATION; ONTOGENY; MICE;

D O I：

10.1016/j.bbrc.2009.01.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as "dedifferentiation" or "transdifferentiation". However, since MSCs have only been defined in vitro, Much of their development in Vivo is Still unknown. Here, we prospectively identified MSCs in the bone marrow from adult transgenic mice encoding neural crest-specific P0-Cre/FIoxed-EGFP and Wnt1-Cre/Floxed-EGFP. EGFP-positive MSCs formed spheres that expressed neural crest stem cell genes and differentiated into neurons, glial cells, and myofibroblasts. Interestingly, we observed MSCs both in the GFP(+) and GFP(-) fraction and found that there were no significant differences in the in vitro characteristics between these two populations. Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1114 / 1119

页数：6

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