C-terminal fluorescence labeling of proteins for interaction studies on the single-molecule level

被引:20
作者
Becker, Christian F. W.
Seidel, Ralf
Jahnz, Michael
Bacia, Kirsten
Niederhausen, Thomas
Alexandrov, Kirill
Schwille, Petra
Goody, Roger S.
Engelhard, Martin
机构
[1] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany
[2] Tech Univ Dresden, Sch Med, Inst Biophys, D-01307 Dresden, Germany
关键词
expressed protein ligation; fluorescent labeling; FRET; proteins; single-molecule studies;
D O I
10.1002/cbic.200500535
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homogeneously labeled protein samples are generated by expressed protein ligation for studying protein interactions. Miniscule amounts of samples are used in single-molecule-sensitive measurements and fluorescence resonance energy transfer experiments. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:891 / 895
页数:5
相关论文
共 26 条
[1]   RAS GENES [J].
BARBACID, M .
ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :779-827
[2]   A sensitive fluorescence monitor for the detection of activated Ras: total chemical synthesis of site-specifically labeled Ras binding domain of c-Raf1 immobilized on a surface [J].
Becker, CFW ;
Hunter, CL ;
Seidel, RP ;
Kent, SBH ;
Goody, RS ;
Engelhard, M .
CHEMISTRY & BIOLOGY, 2001, 8 (03) :243-252
[3]   Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation [J].
Cotton, GJ ;
Muir, TW .
CHEMISTRY & BIOLOGY, 2000, 7 (04) :253-261
[4]   Expressed protein ligation - Method and applications [J].
David, R ;
Richter, MPO ;
Beck-Sickinger, AG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2004, 271 (04) :663-677
[5]   SYNTHESIS OF PROTEINS BY NATIVE CHEMICAL LIGATION [J].
DAWSON, PE ;
MUIR, TW ;
CLARKLEWIS, I ;
KENT, SBH .
SCIENCE, 1994, 266 (5186) :776-779
[6]   Synthesis of fluorescently labeled mono- and diprenylated Rab7 GTPase [J].
Durek, T ;
Alexandrov, K ;
Goody, RS ;
Hildebrand, A ;
Heinemann, I ;
Waldmann, H .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (50) :16368-16378
[7]   Interaction of yeast Rab geranylgeranyl transferase with its protein and lipid substrates [J].
Dursina, B ;
Thomä, NH ;
Sidorovitch, V ;
Niculae, A ;
Iakovenko, A ;
Rak, A ;
Albert, S ;
Ceacareanu, AC ;
Kölling, R ;
Herrmann, C ;
Goody, RS ;
Alexandrov, K .
BIOCHEMISTRY, 2002, 41 (21) :6805-6816
[8]   Protein semi-synthesis in living cells [J].
Giriat, I ;
Muir, TW .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (24) :7180-7181
[9]   Semisynthesis of a glycosylated lm7 analogue for protein folding studies [J].
Hackenberger, CPR ;
Friel, CT ;
Radford, SE ;
Imperiali, B .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (37) :12882-12889
[10]   IDENTIFICATION OF YEAST COMPONENT-A - RECONSTITUTION OF THE GERANYLGERANYLTRANSFERASE THAT MODIFIES YPT1P AND SEC4P [J].
JIANG, Y ;
FERRONOVICK, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (10) :4377-4381