C-terminal fluorescence labeling of proteins for interaction studies on the single-molecule level

被引：20

作者：

Becker, Christian F. W.

Seidel, Ralf

Jahnz, Michael

Bacia, Kirsten

Niederhausen, Thomas

Alexandrov, Kirill

Schwille, Petra

Goody, Roger S.

Engelhard, Martin

机构：

[1] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany

[2] Tech Univ Dresden, Sch Med, Inst Biophys, D-01307 Dresden, Germany

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 06期

关键词：

expressed protein ligation; fluorescent labeling; FRET; proteins; single-molecule studies;

D O I：

10.1002/cbic.200500535

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Homogeneously labeled protein samples are generated by expressed protein ligation for studying protein interactions. Miniscule amounts of samples are used in single-molecule-sensitive measurements and fluorescence resonance energy transfer experiments. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：891 / 895

页数：5

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