A strategic approach to the development of capillary electrophoresis chiral methods for pharmaceutical basic compounds using sulfated cyclodextrins

被引：36

作者：

Zhou, LL ^{[1
]}

Thompson, R ^{[1
]}

Song, S ^{[1
]}

Ellison, D ^{[1
]}

Wyvratt, JM ^{[1
]}

机构：

[1] Merck & Co Inc, Merck Res Labs, Rahway, NJ 07065 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2002年 / 27卷 / 3-4期

关键词：

capillary electrophoresis; sulfated-beta-cyclodextrins; pharmaceutical compounds; method validation; enantiomeric separations;

D O I：

10.1016/S0731-7085(01)00593-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Enantioseparations of basic pharmaceutical compounds were investigated using different types of sulfated cyclodextrins as chiral selectors. A general strategy for method development was described, together with enantiomeric separations of a number of pharmaceutical related compounds. Based on this strategy, systematic method development approaches for several selected compounds were performed by modifying method parameters, such as the concentration of the chiral selectors, buffer pH, type of organic modifiers, buffer type, temperature and applied voltage. The results of the investigation elucidated the separation mechanism. Many practical aspects were also discussed through several specific examples in order to demonstrate how to develop and validate a precise, sensitive, accurate and rugged separation. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：541 / 553

页数：13

共 17 条

[1] Enantiomer separation of drugs by capillary electromigration techniques [J].