Crystallization of the immunodominant outer membrane protein OmpC;: the first protein crystals from Salmonella typhi, a human pathogen

被引：10

作者：

Arockiasamy, A ^{[1
]}

Krishnaswamy, S ^{[1
]}

机构：

[1] Madurai Kamaraj Univ, Sch Biotechnol, Bioinformat Ctr, Madurai 625021, Tamil Nadu, India

来源：

FEBS LETTERS | 1999年 / 453卷 / 03期

关键词：

OmpC; surface antigen; crystallization; X-ray diffraction; Salmonella typhi;

D O I：

10.1016/S0014-5793(99)00746-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

OmpC, a surface antigen of Salmonella typhi was crystallized after several attempts, using PEG 3350. Well shaped hexagonal crystals were grown from vapor diffusion method using octyl glucoside and C12E9 as detergents. Crystals are sensitive to X-ray and diffract weakly up to 7 Angstrom. Porin isoforms, due to the bound lipopolysaccharides, could be the cause for poor diffraction, Crystal quality depends largely on the purification method, and in case of LPS contamination, the genetic background of the bacteria. Crystallization and initial data collection suggest optimum conditions and the method of choice for OmpC crystallization. (C) 1999 Federation of European Biochemical Societies.

引用

页码：380 / 382

页数：3

共 24 条

[1] PREDICTION OF B-CELL EPITOPES FOR SALMONELLA-TYPHI OMPC [J].

AROCKIASAMY, A ;

KRISHNASWAMY, S .

JOURNAL OF BIOSCIENCES, 1995, 20 (02) :235-243

[2]

CALDERON I, 1986, INFECT IMMUN, V52, P209

[3] Structure of the sucrose-specific porin ScrY from Salmonella typhimurium and its complex with sucrose [J].

Forst, D ;

Welte, W ;

Wacker, T ;

Diederichs, K .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (01) :37-46

[4]

GARAVITO R M, 1990, Methods (Orlando), V1, P57, DOI 10.1016/S1046-2023(05)80147-1

[5]

GARAVITO RM, 1986, METHOD ENZYMOL, V125, P309

[6] THE GROWTH AND CHARACTERIZATION OF MEMBRANE-PROTEIN CRYSTALS [J].

GARAVITO, RM ;

MARKOVICHOUSLEY, Z ;

JENKINS, JA .

JOURNAL OF CRYSTAL GROWTH, 1986, 76 (03) :701-709

[7] ISOLATION AND CHARACTERIZATION OF GAL-E MUTANT TY 21A OF SALMONELLA-TYPHI - CANDIDATE STRAIN FOR A LIVE, ORAL TYPHOID VACCINE [J].

GERMANIER, R ;

FURER, E .

JOURNAL OF INFECTIOUS DISEASES, 1975, 131 (05) :553-558

[8] PROTECTION AGAINST SALMONELLA-TYPHI INFECTION IN MICE AFTER IMMUNIZATION WITH OUTER-MEMBRANE PROTEINS ISOLATED FROM SALMONELLA-TYPHI 9,12,D,VI [J].

ISIBASI, A ;

ORTIZ, V ;

VARGAS, M ;

PANIAGUA, J ;

GONZALEZ, C ;

MORENO, J ;

KUMATE, J .

INFECTION AND IMMUNITY, 1988, 56 (11) :2953-2959

[9] EVALUATION OF SINGLE-CRYSTAL X-RAY-DIFFRACTION DATA FROM A POSITION-SENSITIVE DETECTOR [J].

KABSCH, W .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1988, 21 (06) :916-924

[10] Crystallization of OmpC osmoporin from Escherichia coli [J].

Kim, H .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :1399-1400

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