Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.)

被引：98

作者：

Wang, L. H. ^{[1
]}

Zhao, X. L. ^{[1
,2
]}

He, Z. H. ^{[1
,3
]}

Ma, W.

Appels, R.

Pena, R. J.

Xia, X. C. ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[2] Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou 450002, Henan, Peoples R China

[3] CAAS, Int Maize & Wheat Improvement Ctr CIMMYT, China Off, Beijing 100081, Peoples R China

来源：

THEORETICAL AND APPLIED GENETICS | 2009年 / 118卷 / 03期

基金：

美国国家科学基金会;

关键词：

FUNCTIONAL MARKERS; HEXAPLOID WHEAT; BREAD WHEAT; ALLELIC VARIATION; STORAGE PROTEINS; DOUGH PROPERTIES; GROUP-1; CHROMOSOMES; PCR MARKERS; DURUM-WHEAT; RP-HPLC;

D O I：

10.1007/s00122-008-0918-9

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of the end-use products of common wheat. To characterize the LMW-GS genes at the Glu-B3 locus, gene-specific PCR primers were designed to amplify eight near-isogenic lines and Cheyenne with different Glu-B3 alleles (a, b, c, d, e, f, g, h and i) defined by protein electrophoretic mobility. The complete coding regions of four Glu-B3 genes with complete coding sequence were obtained and designated as GluB3-1, GluB3-2, GluB3-3 and GluB3-4. Ten allele-specific PCR markers designed from the SNPs present in the sequenced variants discriminated the Glu-B3 proteins of electrophoretic mobility alleles a, b, c, d, e, f, g, h and i. These markers were validated on 161 wheat varieties and advanced lines with different Glu-B3 alleles, thus confirming that the markers can be used in marker-assisted breeding for wheat grain processing quality.

引用

页码：525 / 539

页数：15

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