Site-specific crosslinking of human microRNPs to RNA targets

被引：10

作者：

Kirino, Yohei ^{[1
]}

Mourelatos, Zissimos ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Dept Pathol & Lab Med, Div Neuropathol, Philadelphia, PA 19104 USA

来源：

RNA | 2008年 / 14卷 / 10期

关键词：

microRNA; miRNA; microRNP; miRNP; RISC; RNAi; Argonaute; Ago;

D O I：

10.1261/rna.1133808

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) regulate the expression of numerous genes and are implicated in the pathogenesis of many human diseases. miRNAs act as specificity determinants to guide deposition of microRNPs (miRNPs) onto miRNA recognition elements (MREs) that are found in mRNA targets. We have adapted a site-specific crosslinking approach, previously used in the analysis of splicing, to interrogate protein factors that physically associate with MREs in human cells. We find that Ago proteins are the only proteins that bind to the MRE in a miRNA-dependent manner. Our method may be used in various experimental systems to analyze protein factors that influence MRE accessibility by miRNAs and the composition of MRE-bound miRNPs.

引用

页码：2254 / 2259

页数：6

共 34 条

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